| 质粒名称: | MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 128607 |
| 存储实验室: | Marvin Tanenbaum |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA4/TO |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 博莱霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
|
插入基因信息 |
|
| 插入基因名称: | 36xMashTag |
| 启动子: | Pcmv |
| 克隆方法: | Gibson克隆 |
| 5’测序引物 | cgcaaatgggcggtaggcgtg |
|
文献引用方法 |
|
| 材料和方法部分: | MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter) was a gift from Marvin Tanenbaum (Addgene plasmid # 128607 ; http://n2t.net/addgene:128607 ; RRID:Addgene_128607) |
| 参考文献部分: | Multi-Color Single-Molecule Imaging Uncovers Extensive Heterogeneity in mRNA Decoding. Boersma S, Khuperkar D, Verhagen BMP, Sonneveld S, Grimm JB, Lavis LD, Tanenbaum ME. Cell. 2019 Jun 5. pii: S0092-8674(19)30499-4. doi: 10.1016/j.cell.2019.05.001. 10.1016/j.cell.2019.05.001 PubMed 31178119 |
MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag repor
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter) 11144 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses 36xMashTag with MoonTag frame as mainframe, followed by
BFP linker and 24 repeats of PP7 hairpins in 3' UTR..
ACCESSION .
VERSION .
KEYWORDS MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11144)
AUTHORS Boersma S, Khuperkar D, Verhagen BMP, Sonneveld S, Grimm JB, Lavis
LD, Tanenbaum ME
TITLE Multi-Color Single-Molecule Imaging Uncovers Extensive Heterogeneity
in mRNA Decoding.
JOURNAL Cell. 2019 Jun 5. pii: S0092-8674(19)30499-4. doi:
10.1016/j.cell.2019.05.001.
PUBMED 31178119
REFERENCE 2 (bases 1 to 11144)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11144
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 820..838
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 841..859
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 869..893
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 984..993
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1025..1081
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1083..1127
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1130..1186
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1188..1232
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1235..1291
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1293..1337
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1340..1396
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1398..1442
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1445..1501
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1503..1547
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1550..1606
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1608..1652
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1655..1711
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1713..1757
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1760..1816
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1818..1862
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1865..1921
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 1923..1967
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1970..2026
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2028..2072
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 2075..2131
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2133..2177
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 2180..2236
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2238..2282
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 2285..2341
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2343..2387
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 2390..2446
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2448..2492
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 2495..2551
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2553..2597
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 2600..2656
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2658..2702
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 2705..2761
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2763..2807
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 2810..2866
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2868..2912
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 2915..2971
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2973..3017
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 3020..3076
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 3078..3122
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 3125..3181
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 3183..3227
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 3230..3286
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 3288..3332
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 3335..3391
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 3393..3437
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 3440..3496
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 3498..3542
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 3581..3637
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 3639..3683
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 3686..3742
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 3744..3788
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 3791..3847
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 3849..3893
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 3896..3952
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 3954..3998
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 4001..4057
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 4059..4103
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 4106..4162
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 4164..4208
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 4211..4267
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 4269..4313
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 4316..4372
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 4374..4418
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 4421..4477
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 4479..4523
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 4584..4628
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 4689..4733
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 4736..4792
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 4794..4838
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
primer_bind complement(7152..7169)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7158..7382
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 7428..7856
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7515..7534)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7725..7746
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7865..7885)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7870..8199
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8050..8185
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8112..8131
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 8247..8294
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 8313..8687
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 8817..8938
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8854..8873)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8908..8927
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8987..9003)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8987..9003)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9000..9022)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9011..9027
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9035..9065)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9080..9101
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9218..9235)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9389..9977)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9469..9488)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10148..11008)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10771..10790
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11009..11113)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaaactaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
961 ctctagcgtt taaacttaag cttgccacca tgggcgccat catacacgcg gccgcaccgg
1021 tcaagaagaa cttctcagca agaattatca tttggagaac gaagtggctc gtctcaagaa
1081 acaagaacga gcaagaatta ctggaattgg acaagtgggc aagcctccag aagaactgct
1141 ttcaaagaat taccacctgg aaaacgaggt tgccagactc aaaaagcaag aacgagcagg
1201 aactcctcga actcgacaag tgggcttctc tccagaggag ttactcagca aaaattatca
1261 tttggagaac gaagttgcac gactcaagaa agaaaaacga acaggagtta ctcgagctcg
1321 acaagtgggc cagcctccag aggagttact ctcgaaaaat tatcatctcg aaaacgaagt
1381 ggctcggctc aaaaagcaag aacgaacaag aactcctcga actggacaag tgggcatctc
1441 tcgagaagag ctattatcca aaaactacca cctcgaaaac gaggtggcac gcctcaaaaa
1501 gcaagaacga gcaagaattg ttggaactcg acaagtgggc aagccttcag aagagctgct
1561 atccaagaat tatcatctcg agaacgaggt ggcgcgtctc aagaaacaag aacgagcaag
1621 aattactcga actcgacaag tgggcttctc tcgagaggaa ctgctctcga agaactatca
1681 tctggaaaac gaggtcgctc gactcaaaaa gcaaaaacga acaggagctg ctggagttgg
1741 acaagtgggc cagcctccag aggaactact ttcaaagaat taccacctcg aaaacgaagt
1801 tgctcgactc aaaaagcaag aacgaacagg aattactcga actcgacaag tgggcatctc
1861 tccagaagaa ttactcagca aaaattatca tctggaaaac gaggtggcga gactcaaaaa
1921 gcaagaacga gcaggaactc ctcgaattgg acaagtgggc aagcctgcag aagagttgct
1981 atcgaaaaat tatcatctgg agaacgaagt tgcaaggctc aaaaaggaaa aacgagcaag
2041 aactactcga actcgacaag tgggcttctc tgcagaggag ttgctctcga aaaactacca
2101 cttggaaaac gaggtcgcga ggctcaaaaa gcaaaaacga acaagagctg ctcgagctgg
2161 acaagtgggc cagcctggag aggagttact cagcaaaaac tatcatctcg agaacgaagt
2221 cgcgcgcctc aagaaagaag aacgaacaag aactactcga actggacaag tgggcatctc
2281 tgcagaagaa ctcttatcga agaactacca cctcgaaaac gaggtcgcca ggctcaaaaa
2341 ggaagaacga gcaggaactg ctggagctgg acaagtgggc ctctcttcag aggaactcct
2401 cagcaagaac taccacttgg agaacgaggt cgcgagactc aagaaagaaa aacgaacaag
2461 agctactcga gctcgacaag tgggcctccc ttcagaggaa ttactcagca agaattatca
2521 tttggagaac gaagtcgcca ggctcaagaa acaaaaacga acaggaatta ctcgagctgg
2581 acaagtgggc aagcctggag aggaattact cagcaaaaac taccacttgg aaaacgaagt
2641 cgccaggctc aaaaaagaaa aacgaacagg agttactgga gctcgacaag tgggcttctc
2701 tgcagaagag ttactcagca aaaattacca cttggagaac gaagtggcaa ggctcaagaa
2761 agaagaacga gcaggaactg ctggagctcg acaagtgggc ctctttgcgg aggagctctt
2821 atcgaagaac taccacctcg agaacgaagt cgcccgcctc aagaaacaaa aacgaacagg
2881 agctactcga gttggacaag tgggcctccc tggagaagag ctcctcagca agaactacca
2941 cttggaaaac gaggtggcac gcctcaagaa acaaaaacga acaagaactc ctcgagttgg
3001 acaagtgggc aagcctcgag aagagttact atccaagaat tatcatctcg agaacgaggt
3061 ggctcgactc aaaaagcaaa aacgaacagg agctcctgga gctcgacaag tgggcttctc
3121 tccagaggaa ctcctgtcga agaactatca tctcgaaaac gaggttgcaa gactcaaaaa
3181 ggaagaacga gcaggaactc ctggagctcg acaagtgggc ctctctggag aggaactact
3241 ctcaaagaat taccacctgg aaaacgaagt tgcacgtctc aagaaacaaa aacgaacagg
3301 agttactgga gctcgacaag tgggcctccc tcgagaagaa ctgctctcaa aaaattatca
3361 tttggaaaac gaggttgcac gtctcaaaaa ggaaaaacga acaagaatta ctcgagctcg
3421 acaagtgggc aagccttcag aggaacttct gtcgaaaaat tatcatctcg agaacgaagt
3481 tgcccgactc aaaaagaaaa aacgaacaag agttactgga gctggacaag tgggcttctc
3541 tggggttcga agcaggggca cgcggtccgg ggttcgaagg gaggaacttc tcagcaagaa
3601 ttatcatttg gagaacgaag tggctcgtct caagaaacaa aaacgaacag gaactcctgg
3661 aattggacaa gtgggcaagc cttcagaaga actgctttca aagaattacc atctggaaaa
3721 cgaggttgcc agactcaaga agcaagaacg agcaggaatt actcgaattg gacaagtggg
3781 cctccctcca gaggagttgc tcagcaagaa ttatcatttg gagaacgaag ttgcacgact
3841 caagaaagaa gaacgaacag gagttactcg agctcgacaa gtgggccagc ctccagagga
3901 attactctcg aaaaattatc atctcgaaaa cgaagtggcc cggctcaaaa agcaagaacg
3961 aacaggaact cctcgagctc gacaagtggg cctccctcga gaggaactat tatccaagaa
4021 ctaccacctc gaaaacgagg tggcacgcct caagaagcaa aaacgaacag gaattgttgg
4081 aactcgacaa gtgggcaagc cttcagagga actactatcc aaaaattatc atctcgagaa
4141 cgaggtggcg cgtctcaaaa agcaaaaacg agcaggaact actggaactc gacaagtggg
4201 cctccctcga gaggaattgc tctcgaagaa ctatcatctg gagaacgagg tcgctcgact
4261 caagaagcaa gaacgaacag gagctgctgg aactggacaa gtgggccagc ctccagaaga
4321 acttctttca aaaaattacc acctcgaaaa cgaagttgcg cgactcaaaa agcaaaaacg
4381 agcaggagct cctcgaactc gacaagtggg catctctcca gaggagttac tcagcaagaa
4441 ttatcatctg gaaaacgagg tggcgagact caaaaagcaa gaacgagcaa gagctcctcg
4501 agctggacaa gtgggcatcc ctgcagaaga gttactcagg aaaaattatc atctggaaaa
4561 cgaagttgca aggctcaaaa aggaagaacg aacaggagct actcgaactc gacaagtggg
4621 catccctgca gaggagctgc tcaggaaaaa ctaccacttg gaaaacgagg tcgcgaggct
4681 caagaagcaa gaacgagcag gaactgctcg agctggacaa gtgggccagc ctggagaaga
4741 gcttctcagc aagaactatc atctcgagaa cgaagtcgcg cgcctcaaga aggaaaaacg
4801 agcaagaact actcgagctc gacaagtggg caagcctgca gggcgtacgg ggaccggtgg
4861 agcgagctca ttcgggagaa catgcacatc aagctataca tggagggcac cgtggacaac
4921 catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatc
4981 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctacgagc
5041 ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag
5101 cagtccttcc ctcagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
5161 ctcaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
5221 agaggggtca acttcacatc caacggccct gtcatgcaga agaaaacact cggctgggag
5281 gccttcaccg agacgctgta ccccgccgac ggcggcctgg aaggcagaaa cgacatggcc
5341 ctcaagctcg tgggcgggag ccatctcatc gcaaacatca agaccacata cagatccaag
5401 aaacccgcaa agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga
5461 atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac
5521 tgcgacctcc caagcaaact ggggcacaaa ctcaatccgc gggggtgagt gagtgagggg
5581 ctagcccggt tgtacaggta ccggatccta aggtacctaa ttgcctagaa aggagcagac
5641 gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat gggctcgctg
5701 gctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga aaggagcaga
5761 cgatatggcg tcgctccctg caggtcgact ctagaaacca gcagagcata tgggctcgct
5821 ggctgcagta ttcccgggtt cattagatcc taaggtacct aattgcctag aaaggagcag
5881 acgatatggc gtcgctccct gcaggtcgac tctagaaacc agcagagcat atgggctcgc
5941 tggctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta gaaaggagca
6001 gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg
6061 ctggctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc
6121 agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc
6181 gctggctgca gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaggag
6241 cagacgatat ggcgtcgctc cctgcaggtc gactctagaa accagcagag catatgggct
6301 cgctggctgc agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaagga
6361 gcagacgata tggcgtcgct ccctgcaggt cgactctaga aaccagcaga gcatatgggc
6421 tcgctggctg cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaagg
6481 agcagacgat atggcgtcgc tccctgcagg tcgactctag aaaccagcag agcatatggg
6541 ctcgctggct gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaag
6601 gagcagacga tatggcgtcg ctccctgcag gtcgactcta gaaaccagca gagcatatgg
6661 gctcgctggc tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa
6721 ggagcagacg atatggcgtc gctccctgca ggtcgactct agaaaccagc agagcatatg
6781 ggctcgctgg ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa
6841 aggagcagac gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat
6901 gggctcgctg gctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga
6961 aaggagcaga cgatatggcg tcgctccctg caggtcgact ctagaaacca gcagagcata
7021 tgggctcgct ggctgcagta ttcccgggtt cattagatct cgcgaagggc gaattcacga
7081 ggcgcgcccg gatcgcgatc gcaatcttaa ttaactcgag tctagagggc ccgtttaaac
7141 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
7201 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
7261 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
7321 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
7381 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
7441 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
7501 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
7561 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
7621 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
7681 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
7741 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
7801 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
7861 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
7921 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
7981 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
8041 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
8101 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
8161 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
8221 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
8281 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
8341 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
8401 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
8461 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
8521 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
8581 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
8641 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
8701 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
8761 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
8821 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
8881 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
8941 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
9001 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
9061 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
9121 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
9181 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
9241 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
9301 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
9361 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
9421 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
9481 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
9541 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
9601 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
9661 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
9721 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
9781 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
9841 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
9901 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
9961 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
10021 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
10081 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
10141 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
10201 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
10261 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
10321 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
10381 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
10441 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
10501 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
10561 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
10621 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
10681 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
10741 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
10801 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
10861 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
10921 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
10981 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
11041 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
11101 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
